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KMID : 0386520150230020005
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Research Institute of Korean Medicone
2015 Volume.23 No. 2 p.5 ~ p.13
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Anti-inflammatory and Antioxidant Effects of Water Extracts of Sasangja-tang(SSJ) and Gami-sasangja-tang(GSJ)
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Choi Jung-Eun
Park Bo-Kyung
Jin Mi-Rim
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Abstract
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Objectives : The anti-inflammatory and antioxidant effects of water extracts of Sasangja-tang(SSJ) and Gami-sasangja-tang(GSJ) were investigated. The effects of SSJ and GSJ were compared.
Methods : We performed cell viability assay in HaCaT cells and RAW 264.7 cells using 3-(4,5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide(MTT) assay. We measured chemokines( regulated on activation normal T-cell expression and secreted ; RANTES/CCL5, interferon-inducible protein; IP-10/CXCL10, macrophage-derived chemokine; MDC/CCL22) in HaCat cells, also we measured cytokines (tumor necrosis factor-α; TNF-α, interleukin-6; IL-6) and nitric oxide(NO) production in RAW 264.7 cells using enzyme-linked immunosorbent assay(ELISA) and NO assay. Western blot assay was used to evaluate the expression for inducible nitric oxide synthase(iNOS) in RAW 264.7 cells.
Results : SSJ and GSJ did not affect the cell viability at the concentrations treated (0-800 μg/ml). As a result of SSJ and GSJ treatment in HaCat cells stimulated by TNF-α(10 ng/ml) and interferon(IFN)-γ(10 ng/ml), the production of RANTES and IP-10 was inhibited significantly. However there was no significant difference in the secretion of MDC. And in RAW 264. 7 cells stimulated by lipopolysaccharide(LPS, 1 μg/ml), SSJ and GSJ treatment significantly inhibited the secretion of TNF-α and IL-6 and the production of NO. The expression of iNOS was also decresed by SSJ and GSJ treatment in RAW 264. 7 cells. Compared with SSJ, GSJ was superior to SSJ in inhibition of RANTES, IP-10, TNF-α, IL-6 and NO production at the concentration of 200 μg/ml.
Conclusion : Both SSJ and GSJ have anti-inflammatory and antioxidant effects. And GSJ has better effects than SSJ.
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KEYWORD
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Sasangja-tang(SSJ) , Gami-sasangja-tang(GSJ) , anti-inflammatory effect , antioxidant effect , cytokine , chemokine
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